Made in USA · ≥99% HPLC GLP-1 SG 10mg
Metabolic Peptide

GLP-1 SG 10mg

GLP-1 analogGLP-1SAcylated incretin

A 31-amino-acid lipidated GLP-1 receptor agonist with three engineered modifications, supplied as 10 mg of sterile lyophilized powder for in vitro laboratory study.

$69.95 25 in stock
For laboratory and research use only. Not for human consumption. This product is not a drug, food or cosmetic. It is sold exclusively to qualified researchers and licensed laboratories.

25 in stock

Tested by Freedom Diagnostics TestingHPLC purity + LC-MS identityLot-matched COA on-site

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COA
Arrives in 3–5 days
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US-synthesizedMade & lab-released in the United States
Independent COAThird-party Certificate of Analysis per lot
HPLC + LC-MSPurity by HPLC, identity by mass spectrometry
Same-day dispatchOrders before 2 PM CT ship the same business day

What is Semaglutide?

Semaglutide is a synthetic, lipidated 31-amino-acid GLP-1 receptor agonist. It binds the GLP-1 receptor with high affinity and resists DPP-4 degradation, which makes it the standard long-acting reference agonist in GLP-1 receptor pharmacology.

Research areas where laboratories buy semaglutide for in vitro work include GLP-1R binding and cAMP signaling, beta-cell insulin-secretion assays and rodent metabolic models, along with receptor-selectivity comparisons against dual and triple incretin agonists and structural studies of the GLP-1R complex.

Every lot, independently verified

Independently third-party tested and matched to the lot ID printed on your vial. Values below are from the latest released lot.
≥99%
HPLC-UV Purity
Passed full QC panel
Independent third-party lab (Freedom Diagnostics Testing)
View COA (PDF)
Per-lot HPLC-UV + LC-MS · independent third-party lab
Lot details
ProductSemaglutide 10 mg
LotPer-batch on COA
AppearanceWhite lyophilized powder
ReleaseFull QC panel per lot
DocumentationLot-matched COA
Full QC panel · third-party lab6 assays
Purity (HPLC-UV)≥99% per lot ✓
Identity (LC-MS)Semaglutide ✓
HPLC conformity (2×)Confirmed ✓
Heavy metals (ICP-MS)Not Detected ✓
SterilityNo Growth ✓
Endotoxin (USP <85>)Within limits ✓
Every lot is released against Koi's full QC panel — HPLC purity, LC-MS identity, heavy metals, sterility, and endotoxin — by an independent third-party laboratory (Freedom Diagnostics Testing). The lot-specific certificate is posted to the public COA library and matched to the lot ID printed on the vial.   Browse full COA library →

Compound information

Chemical & structural reference data — specifications, molecular profile and handling.

Product Specifications
CompoundSemaglutide (synthetic GLP-1 receptor agonist, lipidated)
Vial size10 mg
Purity≥99% by HPLC
Identity confirmationMass spectrometry
FormLyophilized white to off-white hygroscopic powder
Appearance in solutionClear, colorless solution
ManufacturingSynthesized and lyophilized in the United States
Quality testingIndependent third-party laboratory release (Freedom Diagnostics Testing)
Molecular Profile
SequenceHis-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys(2xADO-γGlu-C18 diacid)-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly
Molecular formulaC187H291N45O59
Molecular weight4113.64 g/mol
CAS number910463-68-2
PubChem CID56843331
Other namesNN9535, NNC 0113-0217
Structure class31-amino-acid lipidated GLP-1 analog; non-coded Aib at position 8 (proglucagon numbering); C18 fatty diacid attached via two 8-amino-3,6-dioxaoctanoic acid (ADO) units and a γ-glutamic acid linker to Lys26; Lys-to-Arg substitution at position 34; free C-terminus
Storage & Handling
AppearanceWhite to off-white lyophilized powder (hygroscopic)
SolubilitySterile water or buffer · neutral pH
Storage · powder−20 °C, desiccated, dark (−80 °C long-term)
In solution2–8 °C; aliquot & freeze, avoid freeze-thaw

Semaglutide Mechanism of Action

  • Binds the human GLP-1 receptor (GLP-1R), a class B G-protein-coupled receptor expressed on pancreatic beta-cells, gastric mucosa, and hypothalamic neurons.
  • Activates Gαs-coupled signaling, raising intracellular cyclic AMP (cAMP) and triggering glucose-dependent insulin release from beta-cell models.
  • Suppresses glucagon secretion from pancreatic alpha-cells under hyperglycemic conditions in cell-based assays.
  • Engages GLP-1R on enteric and vagal neurons in rodent models.
  • Engages GLP-1R on hypothalamic and brainstem neurons in animal studies.
  • Resists DPP-4 cleavage due to the Aib-8 substitution, preserving N-terminal integrity required for receptor binding.
  • Binds reversibly to serum albumin through the C18 fatty-diacid side chain, extending its half-life in preclinical pharmacokinetic studies.

Semaglutide Research Applications

  • GLP-1 receptor pharmacology in pancreatic beta-cell lines and HEK293 cells expressing human GLP-1R, where semaglutide serves as the standard long-acting agonist for characterizing binding affinity, EC₅₀ values, and downstream cAMP signaling.
  • Comparative receptor-selectivity studies pairing semaglutide against tirzepatide (dual GLP-1R/GIPR) and retatrutide (triple GLP-1R/GIPR/GCGR) to isolate single-receptor contributions in cell-based reporter assays.
  • Albumin-binding pharmacokinetic research examining how the C18 fatty-diacid side chain prolongs plasma half-life through reversible serum-albumin association.
  • Adipocyte and hepatocyte cell models probing GLP-1R-mediated signaling and lipid-metabolism pathways.
  • Structural biology, including cryo-EM studies of semaglutide bound to the GLP-1 receptor deposited in the Protein Data Bank for docking and structure-activity work.
  • Mechanistic work probing how the Aib substitution at position 8 confers DPP-4 resistance compared with native GLP-1.

Why Buy Semaglutide from Koi Peptides?

  • Every batch of Koi semaglutide is synthesized and lyophilized in the United States, then released against independent third-party laboratory (Freedom Diagnostics Testing) testing.
  • Each lot is tested by HPLC for purity, LC-MS for identity confirmation, and screened for endotoxin before release.
  • The lot ID is printed on every vial and ties back to a public COA library, so researchers can verify documentation before they buy and after the vial arrives.
  • Orders placed before 2 PM CT ship the same business day, and shipping is free on orders over $200.

References

The laboratory and preclinical research behind Semaglutide — cited for scientific context.
J. Medicinal Chemistry
Discovery of the once-weekly GLP-1 analog semaglutide
Lau J. et al. · 2015↗
Molecular Cell
Differential GLP-1R binding and activation by peptide and non-peptide agonists
Zhang X. et al. · 2020↗
Molecular Metabolism
Glucagon-like peptide 1 (GLP-1)
Müller T.D. et al. · 2019↗
View all 6 references +
  1. Lau, J., et al. (2015). Discovery of the once-weekly GLP-1 analog semaglutide. J. Medicinal Chemistry, 58(18), 7370–7380. doi.org/10.1021/acs.jmedchem.5b00726
  2. Deacon, C. F. (2004). Circulation and degradation of GLP-1 and GIP. Regulatory Peptides, 128(2), 117–124. doi.org/10.1016/j.regpep.2004.06.007
  3. Knudsen, L. B., & Lau, J. (2019). The discovery and development of liraglutide and semaglutide. Frontiers in Endocrinology, 10, 155. doi.org/10.3389/fendo.2019.00155
  4. Müller, T. D., et al. (2019). Glucagon-like peptide 1 (GLP-1). Molecular Metabolism, 30, 72–130. doi.org/10.1016/j.molmet.2019.09.010
  5. Coskun, T., et al. (2022). LY3437943, a triple GCGR/GIPR/GLP-1R agonist. Cell Metabolism, 34(9), 1234–1247. doi.org/10.1016/j.cmet.2022.07.013
  6. Zhang, X., et al. (2020). Differential GLP-1R binding and activation by peptide and non-peptide agonists. Molecular Cell, 80(3), 485–500. doi.org/10.1016/j.molcel.2020.09.020

Regulatory Status

Regulatory status — provided for research context.
Research Use

The semaglutide supplied by Koi Peptides is a research-grade reagent synthesized for laboratory research only. It is not formulated, packaged or labeled as a drug, food or cosmetic, and is not authorized for any human or veterinary use.

Disclaimer

For laboratory and research use only. Not for human consumption. This product is not a drug, food or cosmetic. It is sold exclusively to qualified researchers and licensed laboratories. These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

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Frequently Asked Questions About Semaglutide

What is semaglutide used for in research?+
Semaglutide serves as the reference long-acting GLP-1 receptor agonist in pharmacology research. In vitro studies use it to characterize GLP-1R binding affinity, cAMP signaling, and beta-cell insulin release in pancreatic cell lines and HEK293 cells expressing the human receptor. Comparative work pairs it against tirzepatide and retatrutide to isolate single-receptor activity, and structural-biology groups use it in cryo-EM studies of the GLP-1R complex.
What purity is Koi's semaglutide?+
Koi semaglutide is ≥99% pure by HPLC, with identity confirmed by mass spectrometry. Every lot is released against independent third-party laboratory (Freedom Diagnostics Testing) testing, and the COA is publicly available against the lot number printed on the vial.
How do I prepare a semaglutide solution?+
Dissolve in sterile water or a suitable buffer at neutral pH. The lipidated side chain limits solubility between pH 2 and 6, so acidic buffers should be avoided. Prepare working stocks at 1 to 2 mg/mL, then dilute into assay buffer or culture medium immediately before use. For sterile cell-culture work, filter through a 0.22 µm low-protein-binding filter. Store prepared solutions at 2 to 8 °C, or aliquot and freeze at −20 °C or below.
What is the CAS number for semaglutide?+
The CAS number for semaglutide (free base) is 910463-68-2. The PubChem CID is 56843331, and the molecule is also cataloged under the development codes NN9535 and NNC 0113-0217.
Is semaglutide for human use?+
No. Semaglutide is for laboratory and research use only and is not for human consumption. It is sold exclusively to qualified researchers and licensed laboratories.
Does semaglutide come with a Certificate of Analysis?+
Yes. Every lot's Certificate of Analysis covers HPLC purity, LC-MS identity, and endotoxin testing, and is published in our public COA library and matched to the lot ID printed on the vial, so researchers can verify documentation before and after purchase.
How should semaglutide be stored?+
Store the lyophilized powder at −20 °C, desiccated and protected from light; for long-term storage −80 °C is preferred. Semaglutide is hygroscopic, so minimize handling time outside the freezer. Once dissolved, store at 2 to 8 °C or aliquot and freeze. Avoid repeated freeze-thaw cycles, which can promote aggregation in lipidated peptides.
How does semaglutide compare with tirzepatide and retatrutide as a research tool?+
Semaglutide is a single GLP-1 receptor agonist. Tirzepatide is a dual GLP-1R/GIPR agonist, and retatrutide is a triple GLP-1R/GIPR/GCGR agonist. Researchers studying GLP-1R-specific signaling often use semaglutide as the reference single-agonist, adding tirzepatide and retatrutide to isolate the contribution of GIP and glucagon-receptor activation in cell-based and animal models.

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